6EWY
RipA Peptidoglycan hydrolase (Rv1477, Mycobacterium tuberculosis) N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 44.916, 93.960, 138.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.987 - 2.200 |
| R-factor | 0.2303 |
| Rwork | 0.228 |
| R-free | 0.26670 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.987 | 2.270 |
| High resolution limit [Å] | 2.191 | 2.191 |
| Rmerge | 0.056 | 0.381 |
| Rmeas | 0.062 | 0.420 |
| Rpim | 0.026 | 0.174 |
| Number of reflections | 12284 | 614 |
| <I/σ(I)> | 14.5 | 4.1 |
| Completeness [%] | 90.5 | 87.3 |
| Redundancy | 5.8 | 5.3 |
| CC(1/2) | 0.995 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | 277 | 42% MPD, 0.1M MES pH 5.75 |
