6EWC

Crystal structure of non-phosphorylated form of RLS PHOSPHOPEPTIDE BOUND TO HLA-A2 in complex with LILRB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-09
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5417
Spacegroup name	P 32 2 1
Unit cell lengths	117.500, 117.500, 203.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.580 - 3.200
R-factor	0.2076
Rwork	0.206
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7q
RMSD bond length	0.008
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.580	19.580	3.300
High resolution limit [Å]	3.200	10.000	3.200
Rmerge	0.173	0.047	0.442
Rmeas	0.191	0.052	0.489
Total number of observations	149740
Number of reflections	26415	743	2338
<I/σ(I)>	10.73	29.36	3.89
Completeness [%]	96.0	73.3	98.6
Redundancy	5.669	5.829	5.548
CC(1/2)	0.979	0.998	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	12.8% PEG 3350, 3% Tacsimate, 10% dimethyl sulphoxide