6EW4

Human myelin protein P2 F57A mutant, monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-14
Detector	MAR CCD 165 mm
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	112.760, 36.080, 31.170
Unit cell angles	90.00, 96.87, 90.00

Refinement procedure

Resolution	19.112 - 1.271
R-factor	0.1732
Rwork	0.171
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.009
RMSD bond angle	1.199
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2006: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.300
High resolution limit [Å]	1.270	1.270
Rmeas	0.092	0.781
Number of reflections	31892	1880
<I/σ(I)>	8.2	1.2
Completeness [%]	96.8	77.4
Redundancy	2.9	1.8
CC(1/2)	0.996	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	40-42% PEG 6000