6EVN

Crystal structure of peptide-substrate-binding domain of human type II collagen prolyl 4-hydroxylase complex with Pro-Pro-Gly-Pro-Ala-Gly-Pro-Pro-Gly.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 32 2 1
Unit cell lengths	54.926, 54.926, 72.942
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.943 - 1.480
R-factor	0.1647
Rwork	0.163
R-free	0.17880
RMSD bond length	0.007
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	1.510
High resolution limit [Å]	1.480	1.480
Rmerge	0.051	0.915
Rpim	0.018	0.322
Number of reflections	21768	166
<I/σ(I)>	17.6	2.2
Completeness [%]	100.0	100
Redundancy	8.4	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2.45 M ammonium sulphate, 10% DMSO, 5 mM PPGPAGPPG, 5% D-galactose, 100 mM MOPS, pH 6.5