6EVE
Structure of R175A S. cerevisiae Fdc1 with prFMN in the iminium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.480, 98.640, 118.170 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 77.890 - 2.050 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.22602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zac |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.874 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.890 | 2.134 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 1.071 | |
| Number of reflections | 167153 | 15457 |
| <I/σ(I)> | 5.52 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium cacodylate pH 6.5, 0.25M Calcium Acetate, 15% PEG 4k |
