6EV8
Structure of E277Q A. niger Fdc1 with prFMN in the hydroxylated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.910, 63.810, 87.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.080 - 1.030 |
| R-factor | 0.14717 |
| Rwork | 0.146 |
| R-free | 0.16505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4za4 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.595 |
| Data reduction software | iMOSFLM |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.080 | 1.067 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmeas | 0.979 | |
| Number of reflections | 246485 | 14510 |
| <I/σ(I)> | 12.22 | |
| Completeness [%] | 93.0 | |
| Redundancy | 5.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |
