6EUU
The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with a ring-fused 2-pyridone (KSK29)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.973 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.537, 81.180, 59.198 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 45.500 - 2.600 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f1r |
RMSD bond length | 0.003 |
RMSD bond angle | 0.502 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.065 | 0.960 |
Number of reflections | 15110 | |
<I/σ(I)> | 19.3 | 2.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | PrfA was co-crystallized with complex (5 mol excess) using the hanging-drop vapor-diffusion technique. Crystals grew in 5 days after 2 microL of the protein solution (3.2-3.5 mg per ml PrfA, 200 mM NaCl, 20 mM NaP buffer, pH 6.5) was mixed with an equal volume of precipitant solution containing 20-24% PEG-4000, 17% isopropanol, 100 mM Na citrate pH 5.5 and allowed to equilibrate over a 1 ml solution of the precipitant in a Linbro plate (Hampton Research). |