6EUD

Crystal structure of E. coli DExH-box NTPase HrpB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763, 0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.710, 131.410, 166.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.623 - 2.400
R-factor	0.1903
Rwork	0.188
R-free	0.23860
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.087
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.200	1.560
Number of reflections	43551	3189
<I/σ(I)>	11.7	1.79
Completeness [%]	99.9	100
Redundancy	13.1	13.6
CC(1/2)	0.997	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES-NaOH, pH 6.5, 5% [w/v] PEG 6000, 10 % [v/v] MPD, 0.1 M LiCl