6EU6
Sensor Amt Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 99.745, 99.745, 89.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.380 - 1.980 |
| R-factor | 0.15356 |
| Rwork | 0.152 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2h |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.964 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.380 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.112 | 1.461 |
| Rpim | 0.037 | 0.469 |
| Number of reflections | 33401 | 2466 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.3 | 10.5 |
| CC(1/2) | 0.999 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M MES/NaOH pH 7.0 24 % (w/v) of polyethylene glycol 3350 |
