6ETC
Crystal Structure of Human gamma-D-crystallin Mutant P23T+R36S at 1.2 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.020, 31.700, 52.500 |
Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
Resolution | 44.009 - 1.197 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.17450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jgf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.021 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.010 | 1.240 |
High resolution limit [Å] | 1.197 | 1.197 |
Rmerge | 0.077 | 1.140 |
Rmeas | 0.101 | 1.501 |
Number of reflections | 45768 | |
<I/σ(I)> | 8.93 | 1 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 1.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 310 | 0.1M phosphate buffer, 20mM DTT |