6ETA

Crystal Structure of Human Gamma-D crystallin Mutant P23T+R36S at Room Temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	54.036, 82.103, 106.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.165 - 2.198
R-factor	0.2344
Rwork	0.233
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jgf
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.165	2.270
High resolution limit [Å]	2.198	2.198
Rmerge	0.129	1.290
Rmeas	0.139	1.430
Number of reflections	24656
<I/σ(I)>	9.2
Completeness [%]	99.6
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293	0.1M phosphate, 20mM DTT