6ESV
Structure of the phosphate-bound form of AioX from Rhizobium sp. str. NT-26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.544, 124.544, 48.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.070 - 1.780 |
| R-factor | 0.19189 |
| Rwork | 0.190 |
| R-free | 0.22038 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.008 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 88.070 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 36718 |
| <I/σ(I)> | 19.3 |
| Completeness [%] | 99.8 |
| Redundancy | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289.15 | 0.49 M NaH2PO4 and 0.91 M K2HPO4 |
