6ESK
Structure of the apo form of AioX from Rhizobium sp. str. NT-26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.877, 124.877, 47.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.300 - 1.750 |
| R-factor | 0.18854 |
| Rwork | 0.187 |
| R-free | 0.21115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6esv |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.969 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.590 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 38474 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 98.9 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20% PEG 3350, 0.2 M sodium malonate and 0.1 M Bis-Tris propane, pH 8.5 |
