6ESC
Crystal structure of Pseudorabies virus glycoprotein B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 |
| Unit cell lengths | 99.890, 99.890, 272.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.150 - 2.700 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gum |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 2.850 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.074 | 0.523 |
| Rpim | 0.055 | 0.386 |
| Number of reflections | 28086 | |
| <I/σ(I)> | 14.99 | 2.33 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 5.3 | |
| CC(1/2) | 0.980 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 7% PEG 4,000, 0.6M LiCl |
