6ERQ
Structure of the human mitochondrial transcription initiation complex at the HSP promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.620, 197.730, 134.340 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 49.576 - 4.500 |
| R-factor | 0.2932 |
| Rwork | 0.291 |
| R-free | 0.33340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model of the human mitochondrial IC on the LSP promoter |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 4.610 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmeas | 0.087 | 2.232 |
| Number of reflections | 31460 | 2303 |
| <I/σ(I)> | 13.38 | 1.11 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 7 | 7.3 |
| CC(1/2) | 1.000 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | L-Proline, PEG8000, BIS-TRIS |
