6ERQ

Structure of the human mitochondrial transcription initiation complex at the HSP promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	103.620, 197.730, 134.340
Unit cell angles	90.00, 99.39, 90.00

Refinement procedure

Resolution	49.576 - 4.500
R-factor	0.2932
Rwork	0.291
R-free	0.33340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model of the human mitochondrial IC on the LSP promoter
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	4.610
High resolution limit [Å]	4.500	4.500
Rmeas	0.087	2.232
Number of reflections	31460	2303
<I/σ(I)>	13.38	1.11
Completeness [%]	99.1	99.6
Redundancy	7	7.3
CC(1/2)	1.000	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	L-Proline, PEG8000, BIS-TRIS