6ER8
Enterococcus faecalis FIC protein in complex with phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.130, 65.130, 248.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.910 - 2.292 |
| R-factor | 0.2326 |
| Rwork | 0.229 |
| R-free | 0.29870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g03 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.910 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.312 | 1.808 |
| Number of reflections | 23660 | 2338 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 94.8 | 91.7 |
| Redundancy | 4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50mM Tris pH 8.0, 200mM NaCl, 1mM MgCl2, 0.2M Ammonium sulfate, 0.1M Tris-sodium citrate pH 5.6, 15% (w:v) PEG 4000 |
