6ER7

CHEMOTAXIS PROTEIN CHEY FROM Pyrococcus horikoshiI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9334
Spacegroup name	P 1 2 1
Unit cell lengths	53.160, 65.730, 72.890
Unit cell angles	90.00, 110.94, 90.00

Refinement procedure

Resolution	34.038 - 2.620
Rwork	0.212
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tmy
RMSD bond length	0.012
RMSD bond angle	1.652
Data reduction software	xia2 (0.3.8)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0158 2016/10/03)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.000
High resolution limit [Å]	2.600
Rmerge	0.030
Rmeas	0.030
Rpim	0.020
Number of reflections	107515
<I/σ(I)>	38
Completeness [%]	99.5
Redundancy	7.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris-HCl 1.2 M sodium malonate