6EQT
CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE PROTEIN CENP-N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97793 |
| Spacegroup name | P 41 |
| Unit cell lengths | 87.300, 87.300, 81.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.300 - 2.735 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25380 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.261 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.300 | 87.300 | 2.810 |
| High resolution limit [Å] | 2.735 | 12.230 | 2.735 |
| Rmerge | 0.077 | 0.044 | 1.461 |
| Rmeas | 0.082 | 0.047 | 1.551 |
| Total number of observations | 152272 | ||
| Number of reflections | 16182 | 191 | 1172 |
| <I/σ(I)> | 17.28 | 46.41 | 1.37 |
| Completeness [%] | 99.8 | 97.9 | 98.5 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 6-16% PEG3350, pH 6.6-7.2 |
