6EQJ

Crystal Structure of Human Glycogenin-1 (GYG1) Tyr195pIPhe mutant, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	59.030, 101.020, 49.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.967 - 2.180
R-factor	0.2304
Rwork	0.229
R-free	0.26040
RMSD bond length	0.001
RMSD bond angle	0.387
Data reduction software	xia2
Data scaling software	Aimless (0.5.1)
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	101.020	101.020	2.240
High resolution limit [Å]	2.180	9.750	2.180
Rmerge	0.074	0.037	0.771
Rmeas	0.081	0.042	0.933
Rpim	0.033	0.018	0.510
Total number of observations	88786
Number of reflections	15830	225	1042
<I/σ(I)>	12.6
Completeness [%]	98.6	99.7	89.6
Redundancy	5.6	5	2.8
CC(1/2)	0.998	0.998	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293.15	28% PEG smear broad, 0.1M sodium/potassium phosphate pH 6.2, 0.2M sodium chloride