6EQ7
MTH1 in complex with fragment 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.015, 60.592, 66.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.738 - 1.500 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.028 | 0.119 |
| Number of reflections | 44725 | 7090 |
| <I/σ(I)> | 30.79 | 9.53 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 3.4 | 3.15 |
| CC(1/2) | 0.999 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 23 % PEG3350, 0.2 M LI2SO4, 0.1 M SODIUM ACETATE PH4.5 |
