6EP7
ARABIDOPSIS THALIANA GSTU23, GSH bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.070, 50.645, 113.518 |
| Unit cell angles | 90.00, 108.54, 90.00 |
Refinement procedure
| Resolution | 28.842 - 1.947 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 6EP6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.947 | 1.950 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 35062 | |
| <I/σ(I)> | 21.5 | 4.93 |
| Completeness [%] | 94.6 | 85.8 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.16 M MGCL2, 0.08 M TRIS PH 8.5, 24% PEG4000, 20% GLYCEROL |
