6EP3

Lar controls the expression of the Listeria monocytogenes agr system and mediates virulence.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980065, 0.97915
Spacegroup name	P 41
Unit cell lengths	122.445, 122.445, 61.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.290 - 2.200
R-factor	0.2214
Rwork	0.220
R-free	0.24850
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.290	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.116
Rpim	0.055	0.882
Number of reflections	46113	4603
<I/σ(I)>	8.62	0.58
Completeness [%]	99.3	96.92
Redundancy	5.4	5.5
CC(1/2)	0.998	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.5 M lithium sulfate, 100 mM sodium Hepes pH 7.5