6EOK

Crystal structure of E. coli L-asparaginase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	76.330, 121.230, 126.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.495 - 2.500
R-factor	0.1925
Rwork	0.189
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eca
RMSD bond length	0.008
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.650
High resolution limit [Å]	2.500	2.500
Number of reflections	40608	6231
<I/σ(I)>	7.9	2.1
Completeness [%]	98.1	94.6
Redundancy	5.5	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES-NaOH, 10 mM ZnCl2, 25% V/V PEG-MME 550