6ENS
Structure of mouse wild-type RKIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-12 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.580, 54.090, 97.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.12695 |
| Rwork | 0.125 |
| R-free | 0.16809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.559 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.060 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.082 | 0.644 |
| Number of reflections | 42630 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 98.5 | 97.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | PEG 6000, sodium acetate pH 4.6 |
