6EN0

Structure of the Tn1549 transposon Integrase (aa 82-397) in complex with circular intermediate DNA (CI5-DNA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	77.660, 124.320, 77.400
Unit cell angles	90.00, 117.69, 90.00

Refinement procedure

Resolution	46.044 - 2.800
R-factor	0.2283
Rwork	0.227
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6emz
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.044	2.870
High resolution limit [Å]	2.800	2.800
Rmeas	0.125	1.739
Number of reflections	31128	2363
<I/σ(I)>	10.67	0.95
Completeness [%]	96.6	98.5
Redundancy	2.85	2.87
CC(1/2)	0.995	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Na Acetate pH4.6, 30% PEG300