6EMV

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 61
Unit cell lengths	71.082, 71.082, 192.233
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.392 - 2.900
R-factor	0.196476138728
Rwork	0.194
R-free	0.25048
Starting model (for MR)	previously solved structure ( unpublished)
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	XDS (2015-10-15)
Data scaling software	XDS (2015-10-15)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.392	2.999
High resolution limit [Å]	2.900	2.900
Rmerge	0.193	1.135
Rmeas	0.204	1.203
Rpim	0.068	0.398
Number of reflections	12193	1223
<I/σ(I)>	9.88	1.65
Completeness [%]	99.9	99.67
Redundancy	9.1	9.1
CC(1/2)	0.996	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	10% PEG6000, 0.2M LiCl and 0.1M NaAc pH 5.5