6EMU

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 61
Unit cell lengths	71.084, 71.084, 191.958
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.363 - 2.297
R-factor	0.196891010953
Rwork	0.195
R-free	0.22273
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Crystal structure of TkTrm10 construct ( to be deposited)
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS (2015-10-15)
Data scaling software	XDS (2015-10-15)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.363	2.400
High resolution limit [Å]	2.297	2.300
Rmerge	0.113	1.946
Rmeas	0.116	2.000
Rpim	0.025	0.437
Number of reflections	24345	2406
<I/σ(I)>	20.55	1.45
Completeness [%]	99.8	98.12
Redundancy	21	20.4
CC(1/2)	1.000	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	10% PEG6000, 0.2M LiCl and 0.1M NaAc pH 5.5