6EMS

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	65.687, 65.925, 67.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.007 - 1.996
R-factor	0.198
Rwork	0.196
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a7y
RMSD bond length	0.004
RMSD bond angle	0.617
Data reduction software	XDS (2015-10-15)
Data scaling software	XDS (2015-10-15)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.007	2.067
High resolution limit [Å]	1.996	1.996
Rmerge	0.076	2.289
Rmeas	0.078	2.348
Rpim	0.017	0.514
Number of reflections	20375	1976
<I/σ(I)>	24.82	1.1
Completeness [%]	100.0	99.85
Redundancy	19.9	20.1
CC(1/2)	1.000	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	18% PEG3350 and 0.1M Tris pH 7.5