6ELM
Crystal structure of the human WNK2 CCT1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.928 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.197, 46.492, 71.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.944 - 1.140 |
R-factor | 0.1397 |
Rwork | 0.138 |
R-free | 0.16830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o2c |
RMSD bond length | 0.015 |
RMSD bond angle | 1.297 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.490 |
High resolution limit [Å] | 1.140 |
Number of reflections | 35275 |
<I/σ(I)> | 17.3 |
Completeness [%] | 100.0 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 30% PEG1000 -- 0.1M SPG pH 8.0 |