6EKM
Crystal structure of mammalian Rev7 in complex with human Rev3 second binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.200, 48.640, 116.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.400 - 2.760 |
| R-factor | 0.23152 |
| Rwork | 0.230 |
| R-free | 0.25843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ekl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.390 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 2.950 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 5897 | 4231 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.45 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium formate, 0.1 M ammonium acetate, 0.1 M sodium citrate tribasic dehydrate, 0.1 M sodium potassium tartrate tetrahydrate, 0.1 M sodium oxamate, 0.1 M Imidazole - MES monohydrate (acid) pH 6.5 and 12.5% v/v MPD, 12.5% PEG 1000, 12.5% w/v PEG 3350 |
