6EKJ

Crystal structure of mammalian Rev7 in complex with human Chromosome alignment-maintaining phosphoprotein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00002
Spacegroup name	P 21 21 21
Unit cell lengths	47.260, 51.620, 126.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.490 - 1.600
Rwork	0.240
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o8k
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	41580	5973
<I/σ(I)>	26.8
Completeness [%]	100.0	100
Redundancy	11.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	293	1.8 M ammonium sulfate, 0.09 M CAPS/sodium hydroxide pH 10.5, 0.18 M lithium sulfate, 0.009 M sodium nitrate, 0.009 M sodium phosphate dibasic, 0.01 M Tris Bicine pH 8.5, 1.25% v/v MPD, 1.25% PEG 1000, 1.25% w/v PEG 3350