6EJT

Nuclease NucB from Bacillus licheniformis in P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98148
Spacegroup name	P 1 21 1
Unit cell lengths	23.228, 47.821, 100.234
Unit cell angles	90.00, 90.75, 90.00

Refinement procedure

Resolution	47.820 - 1.700
R-factor	0.197
Rwork	0.196
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ejs
RMSD bond length	0.016
RMSD bond angle	1.759
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.031	0.090
Rmeas	0.037	0.114
Rpim	0.026	0.078
Number of reflections	22140	963
<I/σ(I)>	21.8	8.3
Completeness [%]	91.1	75.2
Redundancy	3.1	2.7
CC(1/2)	0.999	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	1.8 M ammonium sulphate, 0.1 M sodium acetate pH 4.6, soaked in 0.02 M 3'-AMP