6EJL

Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-10
Detector	Bruker PHOTON II
Wavelength(s)	1.3418
Spacegroup name	C 1 2 1
Unit cell lengths	74.980, 93.427, 68.491
Unit cell angles	90.00, 99.74, 90.00

Refinement procedure

Resolution	30.322 - 2.382
R-factor	0.1966
Rwork	0.196
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qjb
RMSD bond length	0.008
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.010	2.530
High resolution limit [Å]	2.380	2.380
Rmerge	0.077	0.277
Number of reflections	18601	2937
<I/σ(I)>	20.57	7.15
Completeness [%]	99.4	97.8
Redundancy	6.96	6.89
CC(1/2)	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	Morpheus HT-96, condition H12