6EJL
Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 70 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.980, 93.427, 68.491 |
| Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
| Resolution | 30.322 - 2.382 |
| R-factor | 0.1966 |
| Rwork | 0.196 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.010 | 2.530 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.077 | 0.277 |
| Number of reflections | 18601 | 2937 |
| <I/σ(I)> | 20.57 | 7.15 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 6.96 | 6.89 |
| CC(1/2) | 0.999 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Morpheus HT-96, condition H12 |
