6EIZ
A de novo designed hexameric coiled coil CC-Hex2 with farnesol bound in the channel.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-01-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.080, 126.230, 58.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.345 - 1.850 |
| R-factor | 0.2229 |
| Rwork | 0.221 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pn8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.345 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.036 | 0.416 |
| Number of reflections | 19015 | 19015 |
| <I/σ(I)> | 10.5 | 3.6 |
| Completeness [%] | 92.5 | 57 |
| Redundancy | 11.5 | 11.7 |
| CC(1/2) | 0.997 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M Na HEPES, 4.3 M Sodium Chloride |
