6EI9
Crystal structure of E. coli tRNA-dihydrouridine synthase B (DusB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980872 |
| Spacegroup name | P 43 |
| Unit cell lengths | 108.480, 108.480, 70.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.550 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bfa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.641 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 26834 | 2675 |
| <I/σ(I)> | 18.96 | 0.84 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.8 | 8.1 |
| CC(1/2) | 1.000 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6 M lithium sulfate 100 mM HEPES |
