6EI6
CC2D1B coordinates ESRCT-III activity during the mitotic reformation of the nuclear envelope
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.410, 54.050, 97.900 |
Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
Resolution | 59.594 - 2.461 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.24750 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.216 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.594 | 2.590 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.072 | 0.360 |
Number of reflections | 33151 | |
<I/σ(I)> | 6 | 3.6 |
Completeness [%] | 98.8 | 99.2 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.995 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M Bis-Tris pH 6.5, 15-25% PEG 3500 Mme, 200 mM ammonium sulfate |