6EI3
Crystal structure of auto inhibited POT family peptide transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2013-09-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.96859 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.060, 65.230, 70.340 |
Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
Resolution | 24.650 - 2.100 |
R-factor | 0.1809 |
Rwork | 0.179 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aps |
RMSD bond length | 0.010 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.650 | 24.650 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.097 | 0.032 | 0.686 |
Rmeas | 0.103 | 0.034 | 0.774 |
Rpim | 0.035 | 0.012 | 0.354 |
Total number of observations | 3551 | 12301 | |
Number of reflections | 36377 | 416 | 2655 |
<I/σ(I)> | 15.2 | 51.9 | 2.5 |
Completeness [%] | 99.9 | 94.6 | 99.9 |
Redundancy | 8.2 | 8.5 | 4.6 |
CC(1/2) | 0.999 | 0.999 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8.5 | 292 | 100 mM TrisHCl, 120 mM ammonium tartrate, 20 %(v/v) PEG 400, pH 8.5 |