6EHR

The crystal structure of the human LAMTOR-RagA CTD-RagC CTD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0723
Spacegroup name	P 21 21 21
Unit cell lengths	87.090, 92.448, 127.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.224 - 2.898
R-factor	0.2188
Rwork	0.216
R-free	0.26260
RMSD bond length	0.002
RMSD bond angle	0.419
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.224	3.070
High resolution limit [Å]	2.898	2.900
Rmerge	0.156	0.933
Rpim	0.078	0.478
Number of reflections	21957
<I/σ(I)>	8.2	1.4
Completeness [%]	93.8	93.4
Redundancy	4.4
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% [w/v] PEG 4000, 0.1M tri-sodium-citrate pH5.5, 5% [v/v] isopropanol