6EHP
The crystal structure of the human LAMTOR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 133.896, 133.896, 75.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.19942 |
| Rwork | 0.198 |
| R-free | 0.22758 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 1.440 |
| Rpim | 0.027 | 0.543 |
| Number of reflections | 256155 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.7 | 97.1 |
| Redundancy | 8.2 | |
| CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% [w/v] PEG 3350, 0.1M Tris/HCl pH8.5, 0.2M MgCl2, 3% 2-Methyl-2-propanol |
