6EH8

HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.917
Spacegroup name	P 1 21 1
Unit cell lengths	69.210, 49.500, 72.590
Unit cell angles	90.00, 94.66, 90.00

Refinement procedure

Resolution	52.080 - 2.510
R-factor	0.2266
Rwork	0.223
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gkz
RMSD bond length	0.011
RMSD bond angle	1.552
Data reduction software	xia2
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.084	52.084	2.580
High resolution limit [Å]	2.510	11.230	2.510
Rmerge		0.030	1.388
Rmeas	0.147	0.040	1.706
Rpim	0.073	0.021	0.834
Number of reflections	16674
<I/σ(I)>	12.4	20.7	0.4
Completeness [%]	98.2	97.7	97.9
Redundancy	3.8	3.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% PEG 4K, 15% Glycerol, 100mM MES pH 7.0