6EH5
003 Human T-Cell Receptor specific for HIV GAG epitope SLYNTVATL carried by Human Leukocyte Antigen HLA-A*0201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.150, 81.170, 64.900 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 50.690 - 1.290 |
| R-factor | 0.16957 |
| Rwork | 0.168 |
| R-free | 0.19503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gkz |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.692 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.690 |
| High resolution limit [Å] | 1.290 |
| Number of reflections | 106513 |
| <I/σ(I)> | 16.1 |
| Completeness [%] | 94.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 4K, 200mM Ammonium sulfate, 100mM Sodium cacodylate pH 6.5 |
