6EGU

Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	64.567, 195.729, 65.496
Unit cell angles	90.00, 113.96, 90.00

Refinement procedure

Resolution	37.883 - 2.300
R-factor	0.183
Rwork	0.180
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hj1
RMSD bond length	0.004
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.175	0.689
Rpim	0.111	0.429
Number of reflections	65643
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	291	14.1% (w/v) PEG 5000 MME, 0.1 M Bis-Tris pH 6.2, 0.1M (NH4)2SO4, 1.8 mM UDM, and 5% glycerol. Note: 80 mM 1,2-dipropionyl-sn-glycero-3-phosphocholine added to protein solution