6EGK

T181N Cucumene Synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	300
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	58.330, 91.810, 124.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.480 - 1.960
R-factor	0.1754
Rwork	0.174
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.769
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.480	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.133	1.020
Rpim	0.058	0.468
Number of reflections	47590
<I/σ(I)>	12.9
Completeness [%]	97.5
Redundancy	6.1
CC(1/2)	0.996	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Bis-Tris, 12% PEG monomethyl ether 5000