6EG1

Crystal structure of Dpr2 Ig1-Ig2 in complex with DIP-Theta Ig1-Ig3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	.9792
Spacegroup name	P 21 21 21
Unit cell lengths	116.771, 120.908, 144.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.959 - 2.950
R-factor	0.1803
Rwork	0.178
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eg0
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.000	3.060
High resolution limit [Å]	2.950	2.950
Rmerge	0.121	0.715
Number of reflections	43769	4518
<I/σ(I)>	11.9
Completeness [%]	99.8
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% PEG3350, 0.2 M triammonium citrate, pH 6.5, cryoprotectant: 15% (2R,3R)-(-)-2,3-butanediol