6EFN
Structure of a RiPP maturase, SkfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.462, 83.110, 61.299 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 42.574 - 1.291 |
| R-factor | 0.1445 |
| Rwork | 0.142 |
| R-free | 0.16850 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.574 | 1.340 |
| High resolution limit [Å] | 1.291 | 1.291 |
| Number of reflections | 92230 | |
| <I/σ(I)> | 43.6 | 1.8 |
| Completeness [%] | 96.7 | 76.2 |
| Redundancy | 5.6 | 2.6 |
| CC(1/2) | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294.15 | 0.25 M magnesium formate, 15% PEG3350 |
