6EFK
Crystal structure of the human CHIP TPR domain in complex with a 5mer acetylated HSP70 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.709, 70.342, 77.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.946 - 1.500 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q49 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.844 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.950 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 40943 | 3686 |
| <I/σ(I)> | 9.42 | 1.55 |
| Completeness [%] | 96.6 | 86.34 |
| Redundancy | 11.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.4 M CaCl2, 0.1 M HEPES (pH 7.5), 25% PEG 4000 |
