6EFI
SK678 binding region (Siglec + Unique)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.624, 59.579, 61.832 |
Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
Resolution | 38.718 - 1.715 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.21170 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 45715 | |
<I/σ(I)> | 15 | |
Completeness [%] | 97.7 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M bicine pH 7.6, 25% PEG 6000, 0.005 M hexamine Cobalt chloride |