6EEL

Crystal Structure of Myoferlin C2A with divalent cation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	CCD
Collection date	2015-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1271
Spacegroup name	P 21 21 21
Unit cell lengths	47.350, 83.110, 94.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.340 - 1.930
R-factor	0.1857
Rwork	0.181
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dmh
RMSD bond length	0.017
RMSD bond angle	1.853
Data reduction software	DENZO
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.345	62.345	2.030
High resolution limit [Å]	1.929	6.100	1.930
Rmerge	0.109	0.034	0.645
Rmeas	0.135	0.041	0.777
Rpim	0.060	0.019	0.348
Number of reflections	28742	1019	4165
<I/σ(I)>	9.5	13.7	1.1
Completeness [%]	99.8	99.6	99.9
Redundancy	4.8	4.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		310	orthorhombic shaped crystals