6EDK
Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis with N10-formyltetrahydrofolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.200, 77.300, 40.460 |
Unit cell angles | 90.00, 103.90, 90.00 |
Refinement procedure
Resolution | 47.180 - 1.800 |
R-factor | 0.1706 |
Rwork | 0.168 |
R-free | 0.19380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ci2 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.353 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.180 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 | 0.763 |
Rpim | 0.027 | |
Number of reflections | 26389 | 1627 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 97.9 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M ammonium sulfate, 0.1M MES pH 6.5, 26% PEG5000 MME |