6EDC

hcGAS-16bp dsDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	99.813, 99.813, 238.644
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	86.441 - 2.712
R-factor	0.2409
Rwork	0.239
R-free	0.27760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k96
RMSD bond length	0.004
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.441	2.800
High resolution limit [Å]	2.710	2.710
Number of reflections	19571
<I/σ(I)>	11.3
Completeness [%]	99.1	96.1
Redundancy	12
CC(1/2)	0.996	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M NaCl, 0.1 M HEPES, pH 7.5, and 28% PEG400 (v/v)